Transformation and transfection of anthracycline-producing streptomycetes
نویسندگان
چکیده
منابع مشابه
compactifications and representations of transformation semigroups
this thesis deals essentially (but not from all aspects) with the extension of the notion of semigroup compactification and the construction of a general theory of semitopological nonaffine (affine) transformation semigroup compactifications. it determines those compactification which are universal with respect to some algebric or topological properties. as an application of the theory, it is i...
15 صفحه اولTransduction of plasmid DNA in macrolide producing streptomycetes.
Transduction was mediated by a 8-kb segment of bacteriophage FP43 DNAcloned into plasmid pIJ702: The resulting plasmid, pRHBlOl, was packaged in bacteriophage heads as linear concatemers and was transduced into about 80% of the Streptomyces species tested1}. We showed that the efficiency of transduction can vary markedly depending on the cell growth conditions, and that under optimum growth con...
متن کاملMolecular detection of streptomycin-producing streptomycetes in Brazilian soils.
Actinomycetes were isolated from soybean rhizosphere soil collected as two field sites in Brazil. All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultiv...
متن کاملrapid isolation and molecular detection of streptomycin-producing streptomycetes
introduction: streptomyces species are mycelial, aerobic gram-positive bacteria that are isolated from soil and produce a diverse range of antibiotics. streptomyces griseus produces the antibiotic, streptomycin and forms spores even in a liquid culture. the gene cluster for the production of streptomycin antibiotic contains strr gene that encodes strr, a pathway-specific regulator. then, this p...
متن کاملTransformation and transfection of Pseudomonas aeruginosa: effects of metal ions.
The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 promoted transfection of P. aeruginosa by the linear deoxyribonucleic acid of ph...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1986
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.51.1.126-131.1986